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How does sodium dodecyl sulfate gel electrophoresis ( SDS-PAGE ) work?
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique used to move charged molecules through a gel matrix by means of an electric current. This procedure is used to determine protein subunit composition, verify homogeneity of the protein sample, and purify proteins for use in other applications.
How are proteins separated by SDS-PAGE transferred?
Proteins separated by SDS-PAGE are “transferred” from the polyacrylamide gel to a membrane, using a specialized apparatus (blotting apparatus). Blotting apparatus A semi-dry or a tank system can be used for transfer. Comparison between blotting apparatuses Semi-dry system Tank system Blotting time Short Long Buffer volume Small Large
Which is better SDS PAGE or SDS-PAGE?
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Last Update: Oct 2021
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